Plasmid_Backbone

Part:BBa_K879918:Design

Designed by: Nien-Tsu Tseng   Group: iGEM12_Alberta   (2012-10-01)


TetO ori


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2053
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2059
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2053
    Illegal XhoI site found at 1037
    Illegal XhoI site found at 1929
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2053
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2053
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2068
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

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Source

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References